![]() the spatial distribution of cells within a region of interest). ![]() It has been the experience of the authors that most neuroscientists potentially interested in design-based stereology need to analyze volumes of brain regions, numbers of cells (neurons, glial cells) within these brain regions, mean volumes (nuclear, perikaryal) of these cells, length densities of linear biological structures such as vessels and nerve fibers within brain regions, and the cytoarchitecture of brain regions (i.e. Rather, it is written by users for users, provides the reader with a guided tour through the relevant literature. It does not comprise a full description of all stereologic methods available. The objective of this review is to provide some clarification. ![]() However, much confusion and uncertainty remains about the meaning, implications, and advantages of these design-based stereologic methods. In particular, several new approaches, known as design-based stereologic methods, have become available and have been successfully applied to neuromorphological research. Quantitative morphology of the CNS has recently undergone major developments.
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